3,3',5,5'-Tetramethylbenzidine

Product Information

Molecular Formula:
C16H20N2
Molecular Weight:
240.35
Description
3,3',5,5'-Tetramethylbenzidine is a new type safe chromogenic substrate used in staining procedure in immunohistochemistry. As a new zymolyte of peroxidase, TMB has been widely used in enzyme-immunoassay (EIA) and enzyme-linked-immunosorbent serological assay (ELISA).
Synonyms
3,3',5,5'-Tetramethyl-[1,1'-biphenyl]-4,4'-diamine; TMB; 3,5,3',5'-Tetramethylbenzidine; 4,4'-Diamino-3,3',5,5'-tetramethylbiphenyl; 3,3',5,5'-tetramethylbiphenyl-4,4'-diamine; Tetramethylbenzidine
IUPAC Name
4-(4-amino-3,5-dimethylphenyl)-2,6-dimethylaniline
Canonical SMILES
CC1=CC(=CC(=C1N)C)C2=CC(=C(C(=C2)C)N)C
InChI
InChI=1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3
InChI Key
UAIUNKRWKOVEES-UHFFFAOYSA-N
Boiling Point
368.6 °C at 760 mmHg
Melting Point
168-171 °C
Purity
≥ 99 %
Density
1.071 g/cm3
Solubility
Slightly soluble in DMSO, Methanol
Appearance
White crystal powder
Application
Used as a clinical reagent for testing blood.
Storage
2-8 °C under inert atmosphere
Refractive Index
1.5519 (estimate)
LogP
4.91400
Vapor Pressure
0.0000005 [mmHg]
Decomposition
When heated to decomp it emits toxic fumes of /nitrogen oxides

Safety Information

Hazards
H315:
Causes skin irritation.
H319:
Causes serious eye irritation.
H413:
May cause long-lasting harmful effects to aquatic life.
Precautionary Statement
P261, P264, P270, P271, P280, P301+P312, P302+P352, P304+P340, P305+P351+P338, P312, P321, P330, P332+P313, P337+P313, P362, P403+P233, P405, and P501
Signal Word
Warning

Computed Properties

XLogP3
3.6
Hydrogen Bond Donor Count
2
Hydrogen Bond Acceptor Count
2
Rotatable Bond Count
1
Exact Mass
240.162648646 g/mol
Monoisotopic Mass
240.162648646 g/mol
Topological Polar Surface Area
52Ų
Heavy Atom Count
18
Formal Charge
0
Complexity
226
Isotope Atom Count
0
Defined Atom Stereocenter Count
0
Undefined Atom Stereocenter Count
0
Defined Bond Stereocenter Count
0
Undefined Bond Stereocenter Count
0
Covalently-Bonded Unit Count
1
Compound Is Canonicalized
Yes

Patents

Publication Number Title Priority Date
CN-114113634-A ELISA detection kit for detecting African swine fever virus antibody and application of protein L 2022-01-24
CN-114075565-A Bifunctional G-quadruplex allosteric biosensor for detecting beta-lactoglobulin 2022-01-19
CN-114028559-A Aluminum-manganese composite nanocrystalline and preparation method and application thereof 2021-12-28
CN-114113065-A Flexible noninvasive eye patch type wearable sensor and application thereof 2021-12-27
CN-114113066-A Application of maltol iron peroxide mimic enzyme in detecting hydrogen peroxide and total antioxidant capacity 2021-12-27
JP-2022043219-A Monoclonal antibodies that react with glycopeptides and their uses 2021-12-24
CN-113984755-A Quick detection powder for residual chlorine, ozone and total chlorine in water and preparation method thereof 2021-12-23
CN-114184789-A Prostate specific antigen detection probe and prostate specific antigen detection kit 2021-12-23
CN-114177290-A Nitrogen-doped carbon dot/lithium iron phosphate heterojunction, preparation method and application thereof 2021-12-21
CN-114106354-A Nanocomposite capable of in-situ autocatalytic generation of hydrogen peroxide and sustained release of NO and free radicals, and preparation method and application thereof 2021-12-20

Literatures

PMID Publication Date Title Journal
23026260 2012-11-02 Sensitive and simple determination of bromate in foods disinfected with hypochlorite reagents using high performance liquid chromatography with post-column derivatization Journal of chromatography. A
22986129 2012-10-17 Investigating the interactions between cations, peroxidation substrates and G-quadruplex topology in DNAzyme peroxidation reactions using statistical testing Analytica chimica acta
22900262 2012-10-07 BSA-templated MnO2 nanoparticles as both peroxidase and oxidase mimics The Analyst
22776177 2012-10-01 DNAzyme-functionalized Pt nanoparticles/carbon nanotubes for amplified sandwich electrochemical DNA analysis Biosensors & bioelectronics
22846601 2012-10-01 Isoniazid as a substrate and inhibitor of myeloperoxidase: identification of amine adducts and the influence of superoxide dismutase on their formation Biochemical pharmacology
22829469 2012-09-10 From monomers to π stacks, from nonconductive to conductive: syntheses, characterization, and crystal structures of benzidine radical cations Chemistry (Weinheim an der Bergstrasse, Germany)
22683464 2012-08-01 Identification of a 2-cys peroxiredoxin as a tetramethyl benzidine-hydrogen peroxide stained protein from the thylakoids of the extreme halophyte Arthrocnemum macrostachyum L Plant physiology and biochemistry : PPB
22627993 2012-07-07 Graphene oxide-Fe3O4 magnetic nanocomposites with peroxidase-like activity for colorimetric detection of glucose Nanoscale
22702236 2012-07-03 Colorimetric detection of urine glucose based ZnFe2O4 magnetic nanoparticles Analytical chemistry
22490466 2012-07-01 Development of a near-infrared fluorescence ELISA method using tyramide signal amplification Analytical biochemistry
The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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