CHAPS

Catalog Number Size Price Stock Quantity
B1370-300063 50 g $998 In stock
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Product Information

Molecular Formula:
C32H58N2O7S
Molecular Weight:
614.88
Description
CHAPS is a zwitterionic nondenaturing detergent for solubilizing membrane proteins. It is often used as a detergent in the solubilization and purification of membrane proteins for several advantageous reasons. It is also useful in ion exchange chromatography and isoelectric focusing as it is zwitterionic and does not exhibit a net charge between pH 2 to 12.
Synonyms
N,N-Dimethyl-N-(3-sulfopropyl)-3-[[(3α,5β,7α,12α)-3,7,12-trihydroxy-24-oxocholan-24-yl]amino]-1-propanaminium Inner Salt; 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate
IUPAC Name
3-[dimethyl-[3-[[(4R)-4-[(3R,5S,7R,8R,9S,10S,12S,13R,14S,17R)-3,7,12-trihydroxy-10,13-dimethyl-2,3,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydro-1H-cyclopenta[a]phenanthren-17-yl]pentanoyl]amino]propyl]azaniumyl]propane-1-sulfonate
Canonical SMILES
CC(CCC(=O)NCCC[N+](C)(C)CCCS(=O)(=O)[O-])C1CCC2C1(C(CC3C2C(CC4C3(CCC(C4)O)C)O)O)C
InChI
InChI=1S/C32H58N2O7S/c1-21(8-11-29(38)33-14-6-15-34(4,5)16-7-17-42(39,40)41)24-9-10-25-30-26(20-28(37)32(24,25)3)31(2)13-12-23(35)18-22(31)19-27(30)36/h21-28,30,35-37H,6-20H2,1-5H3,(H-,33,38,39,40,41)/t21-,22+,23-,24-,25+,26+,27-,28+,30+,31+,32-/m1/s1
InChI Key
UMCMPZBLKLEWAF-BCTGSCMUSA-N
Melting Point
156-158°C (dec.)
Flash Point
320 °F
Purity
≥98%
Density
1.01 g/mL at 20°C
Solubility
Slightly soluble in Methanol, Water
Appearance
White to Off-white Solid
Application
Used as a non-denaturing biological detergent; CHAPS is a zwitterionic detergent used in the laboratory to solubilize biological macromolecules such as proteins. It is used as a non-denaturing solvent in the process of protein purification and is especially useful in purifying membrane proteins, which are often sparingly soluble or insoluble in aqueous solution due to their native hydrophobicity.
Shelf Life
1 Year
Storage
-20°C under inert atmosphere

Safety Information

Hazards
H315:
Causes skin irritation.
H319:
Causes serious eye irritation.
Precautionary Statement
P264:
Wash thoroughly after handling.
P280:
Wear protective gloves/protective clothing/eye protection/face protection.
P302+P352:
IF ON SKIN:
Wash with plenty of soap and water.
P332+P313:
If skin irritation occurs:
Get medical advice/attention.
P337+P313:
If eye irritation persists:
Get medical advice/attention.
P362+P364:
Take off contaminated clothing. [As modified by IV ATP].
And wash it before reuse. [Added by IV ATP].

Computed Properties

XLogP3
2.9
Hydrogen Bond Donor Count
4
Hydrogen Bond Acceptor Count
7
Rotatable Bond Count
11
Exact Mass
614.39647337 g/mol
Monoisotopic Mass
614.39647337 g/mol
Topological Polar Surface Area
155Ų
Heavy Atom Count
42
Formal Charge
0
Complexity
1030
Isotope Atom Count
0
Defined Atom Stereocenter Count
11
Undefined Atom Stereocenter Count
0
Defined Bond Stereocenter Count
0
Undefined Bond Stereocenter Count
0
Covalently-Bonded Unit Count
1
Compound Is Canonicalized
Yes

Patents

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Literatures

PMID Publication Date Title Journal
22902529 2012-10-21 Fabrication of an octadecylated silica monolith inside a glass microchip for protein enrichment The Analyst
22931609 2012-08-01 [A novel method for studying nuclear localization signal-mediated nuclear translocation] Nan fang yi ke da xue xue bao = Journal of Southern Medical University
22824320 2012-07-01 Modulation of the neurotensin solution structure in the presence of ganglioside GM1 bicelle Biophysical chemistry
22440890 2012-04-15 Overcoming non-specific adsorption issues for AZD9164 in human urine samples: consideration of bioanalytical and metabolite identification procedures Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
22426713 2012-01-01 High-throughput screening method for lipases/esterases Methods in molecular biology (Clifton, N.J.)
22808001 2012-01-01 Biochemical and physical characterisation of urinary nanovesicles following CHAPS treatment PloS one
22034842 2011-12-15 Lipid and membrane mimetic environments modulate spin label side chain configuration in the outer membrane protein A The journal of physical chemistry. B
21951169 2011-12-01 Identification of Bax-voltage-dependent anion channel 1 complexes in digitonin-solubilized cerebellar granule neurons Journal of neurochemistry
21298564 2011-10-01 Secondary structure and 1H, 13C, 15N resonance assignments of the Golgi-specific PH domain of FAPP1 Biomolecular NMR assignments
21667919 2011-08-02 Behavior of the amphiphile CHAPS alone and in combination with the biopolymer inulin in water and isopropanol-water media Langmuir : the ACS journal of surfaces and colloids
The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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