3,3'-Diaminobenzidine

Product Information

Molecular Formula:
C12H14N4
Molecular Weight:
214.27
Description
3,3'-Diaminobenzidine (CAS# 91-95-2) is a peroxidase substrate and a reagent for spectrophotometric determination of selenium.
Synonyms
4-(3,4-diaminophenyl)benzene-1,2-diamine
IUPAC Name
4-(3,4-diaminophenyl)benzene-1,2-diamine
Canonical SMILES
C1=CC(=C(C=C1C2=CC(=C(C=C2)N)N)N)N
InChI
InChI=1S/C12H14N4/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8/h1-6H,13-16H2
InChI Key
HSTOKWSFWGCZMH-UHFFFAOYSA-N
Boiling Point
481.7 °C at 760 mmHg
Melting Point
177 °C
Flash Point
>200 °C
Purity
> 98.0 % (HPLC)
Density
1.307 g/cm3
Solubility
Solubility in water: very poor
Appearance
White to brown powder to crystal
Application
Used as a reagent.
Storage
0-10 °C
LogP
4.00720

Safety Information

Hazards
H341:
Suspected of causing genetic defects.
H350:
May cause cancer.
Precautionary Statement
P201, P202, P281, P308+P313, P405, and P501
Signal Word
Danger

Computed Properties

XLogP3
0.9
Hydrogen Bond Donor Count
4
Hydrogen Bond Acceptor Count
4
Rotatable Bond Count
1
Exact Mass
214.121846464 g/mol
Monoisotopic Mass
214.121846464 g/mol
Topological Polar Surface Area
104Ų
Heavy Atom Count
16
Formal Charge
0
Complexity
209
Isotope Atom Count
0
Defined Atom Stereocenter Count
0
Undefined Atom Stereocenter Count
0
Defined Bond Stereocenter Count
0
Undefined Bond Stereocenter Count
0
Covalently-Bonded Unit Count
1
Compound Is Canonicalized
Yes

Patents

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CN-114099684-A Application of miR-32-5p in preparation of medicine for improving sensitivity of tumor cells to dihydroartemisinin 2022-01-25
CN-114047337-A Molecular marker related to prognosis of hepatocellular carcinoma patient, immunohistochemical kit and application of molecular marker 2021-12-27
CN-114162444-A Prawn baculovirus detection kit and method thereof 2021-12-08
CN-114053287-A Application of aspirin in preparation of medicine for preventing and treating recurrent abortion caused by unknown reasons 2021-12-01
CN-113924945-A African agapanthus cultivation medium and preparation method thereof 2021-11-25
CN-114066874-A Histopathology image classification method based on color deconvolution and self-attention model 2021-11-25
CN-113813279-A Application of astragalin in resisting aging and inflammation aging 2021-11-23
CN-114182035-A Target nucleic acid instant detection method based on distance signal output 2021-11-17
CN-114002260-A DPC ceramic copper-plated substrate binding force detection method 2021-11-04
CN-113999818-A Immunochromatographic test strip for diagnosing echinococcosis based on detection of circulating antigen 2021-11-03

Literatures

PMID Publication Date Title Journal
23027015 2013-01-01 Photooxidation technology for correlative light and electron microscopy Methods in molecular biology (Clifton, N.J.)
23027016 2013-01-01 Electron microscopy of endocytic pathways Methods in molecular biology (Clifton, N.J.)
23027018 2013-01-01 Cytochemical detection of peroxisomes and mitochondria Methods in molecular biology (Clifton, N.J.)
22863602 2012-11-01 Effect of smoking on the functional aspects of sperm and seminal plasma protein profiles in patients with varicocele Human reproduction (Oxford, England)
22984898 2012-11-01 Energy filtering transmission electron microscopy immunocytochemistry and antigen retrieval of surface layer proteins from Tannerella forsythensis using microwave or autoclave heating with citraconic anhydride Biotechnic & histochemistry : official publication of the Biological Stain Commission
22915187 2012-09-28 Protein-templated gold nanoclusters: size dependent inversion of fluorescence emission in the presence of molecular oxygen Nanoscale
22959769 2012-09-28 Development of a quantitative diagnostic method of estrogen receptor expression levels by immunohistochemistry using organic fluorescent material-assembled nanoparticles Biochemical and biophysical research communications
22610462 2012-08-01 Chromogen-based immunohistochemical method for elucidation of the coexpression of two antigens using antibodies from the same species The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
22595304 2012-07-15 Plant growth stimulation in Prunus species plantlets by BTH or OTC treatments under in vitro conditions Journal of plant physiology
22571419 2012-07-01 Compartment-specific antioxidative defense in Arabidopsis against virulent and avirulent Pseudomonas syringae Phytopathology
The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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