Poly-D-lysine hydrobromide

Product Information

Molecular Formula:
D-Lys-(D-Lys)n-D-Lys · xHBr
Molecular Weight:
227.1
Description
Poly-D-lysine hydrobromide is used for the following applications:• Used in animal cell culture• Rat cortical neuron cultures• Cell culture and siRNA treatment • Primary cell cultures and adult neuronal network model• Cortical astrocyte culture• Immunofluorescence staining (HDF cells were plated onto poly-D lysine (P0899, Sigma-Aldrich) coated glass coverslips)• NPC collection and culture conditions
Poly-D-lysine polymers can be used in preparing surfaces for cell attachment. The D-lysine polymers can also be used with cells that digest poly-L-lysine polymers and cause an excessive uptake of L-lysine. This product is recommended as a cell culture substratum when using 0.5 - 1.0 mL of a 0.1 mg/mL solution to coat 25 cm2. Lower molecular weight versions of the product are less viscous, but high more molecular weight versions provide more attachment sites per molecule.
Synonyms
Poly-D-lysine HBr; d-lysine hydrobromide; (2R)-2,6-diaminohexanoic acid; hydrobromide
IUPAC Name
(2R)-2,6-diaminohexanoic acid;hydrobromide
Canonical SMILES
C(CCN)CC(C(=O)O)N.Br
InChI
InChI=1S/C6H14N2O2.BrH/c7-4-2-1-3-5(8)6(9)10;/h5H,1-4,7-8H2,(H,9,10);1H/t5-;/m1./s1
InChI Key
MEXAGTSTSPYCEP-NUBCRITNSA-N
Flash Point
Not applicable
Storage
−20°C

Computed Properties

Hydrogen Bond Donor Count
4
Hydrogen Bond Acceptor Count
4
Rotatable Bond Count
5
Exact Mass
226.03169 g/mol
Monoisotopic Mass
226.03169 g/mol
Topological Polar Surface Area
89.3Ų
Heavy Atom Count
11
Formal Charge
0
Complexity
106
Isotope Atom Count
0
Defined Atom Stereocenter Count
1
Undefined Atom Stereocenter Count
0
Defined Bond Stereocenter Count
0
Undefined Bond Stereocenter Count
0
Covalently-Bonded Unit Count
2
Compound Is Canonicalized
Yes

Patents

Publication Number Title Priority Date
CN-110982788-A Method for inducing transdifferentiation of satellite glial cells into neurons 2019-12-25
CN-110982788-B Method for inducing transdifferentiation of satellite glial cells into neurons 2019-12-25
WO-2021045182-A1 Tryptase activity measurement substrate 2019-09-05
WO-2020263772-A1 Cell scaffold comprising an electronic circuit 2019-06-24
CN-113613633-A Fusogenic liposome compositions for CNS delivery 2018-11-14
CN-113631718-A Compositions and methods for compartment-specific cargo delivery 2018-11-14
CN-112955174-A Fusogenic liposome compositions and uses thereof 2018-07-09
CN-112272706-A Compositions and methods for membrane protein delivery 2018-02-17
CN-111655292-A Cellular biologicals and therapeutic uses thereof 2017-12-07
CN-107119010-B Synthetic adhesion culture medium for cell culture and preparation method thereof 2017-07-14
The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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