Nile Blue Acrylamide

Product Information

Molecular Formula:
C23H22N3O2CI
Molecular Weight:
408
Description
Nile Blue dye itself is a fluorescent dye that is used routinely in histology to impart a blue color to cell nuclei, where it highlights the distinction between neutral lipids (triglycerides, etc.) which are stained pink and fatty acids, which are stained blue.Nile Blue Acrylamide has been used to covalently link Nile Blue dye into the backbone of polymers for use in various sensor applications. Chandrasekharan, et al have developed a plasticizer-free autoclavable ratiometric alcohol sensor based on Nile Blue methacrylamide incorporated into a hydrogel polymer backbone.1 The film formed from the Nile Blue hydrogel exhibits both absorption and fluorescent changes upon contact with ethanol, and the fluorescences Lmax decreases with the increase of ethanol concentration. The sensor can therefore be used to accurately determine ethanol concentration in a system.In addition, Pham, et al have developed a polymeric nanostructured material incorporating Nile Blue methacrylamide into the polymer, and this material is being developed for the recording of biometric features for anti-counterfeiting applications.2UV Absorption and fluorescence emission maxima of Nile Blue in different solvents3
IUPAC Name
diethyl-[5-(prop-2-enoylamino)benzo[a]phenoxazin-9-ylidene]azaniumchloride
Canonical SMILES
CC[N+](=C1C=CC2=NC3=C(C=C(C4=CC=CC=C43)NC(=O)C=C)OC2=C1)CC.[Cl-]
InChI
InChI=1S/C23H21N3O2.ClH/c1-4-22(27)24-19-14-21-23(17-10-8-7-9-16(17)19)25-18-12-11-15(13-20(18)28-21)26(5-2)6-3/h4,7-14H,1,5-6H2,2-3H31H
InChI Key
DSLXBAJBQIYISM-UHFFFAOYSA-N
Storage
Protect from light. Store at -20°C.

Safety Information

Hazards
Irritant

Computed Properties

Hydrogen Bond Donor Count
1
Hydrogen Bond Acceptor Count
4
Rotatable Bond Count
4
Exact Mass
407.1400546 g/mol
Monoisotopic Mass
407.1400546 g/mol
Topological Polar Surface Area
53.7Ų
Heavy Atom Count
29
Formal Charge
0
Complexity
771
Isotope Atom Count
0
Defined Atom Stereocenter Count
0
Undefined Atom Stereocenter Count
0
Defined Bond Stereocenter Count
0
Undefined Bond Stereocenter Count
0
Covalently-Bonded Unit Count
2
Compound Is Canonicalized
Yes
The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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